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Energetic Manipulation of Chloroplast Protein Import and the Use of Chemical Cross-Linkers to Map Protein–Protein Interactions

  • Hitoshi Inoue
  • Fei Wang
  • Takehito Inaba
  • Danny J. Schnell
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 774)

Abstract

Most chloroplast proteins are synthesized in the cytosol as preproteins with N-terminal cleavable transit peptides and are imported into the organelle through the TOC–TIC translocon system. Import involves a complex set of recognition and membrane translocation steps that ensure the fidelity and unidirectional transport of the polypeptide across the double-membrane chloroplast envelope. To understand the mechanism of import, the molecular interactions and energetics of each step must be defined. Here, we describe the methods for capturing intermediates in the import process through the manipulation of the energy state of chloroplasts, and the use of two different chemical cross-linking approaches to examine the molecular interactions that mediate the import process and to assess the assembly state of the translocons. These approaches can be employed to identify sequential protein–protein interactions, and thereby dissect the pathway and roles of import components during protein import into chloroplasts.

Key words

Chloroplast Import TOC TIC GTP ATP Cross-link Early import intermediate 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Hitoshi Inoue
    • 1
  • Fei Wang
    • 1
  • Takehito Inaba
    • 1
  • Danny J. Schnell
    • 1
  1. 1.Department of Biochemistry and Molecular BiologyUniversity of MassachusettsAmherstUSA

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