Abstract
Seed dormancy and germination are regulated by several plant hormones, such as abscisic acid, gibberellin, auxin (indole-3-acetic acid), ethylene, and brassinosteroid. Endogenous concentrations of a hormone are determined by the balance between biosynthesis and deactivation, and contribute to the regulation of physiological responses. Therefore, profiling of all hormones and their metabolites (hormonome) is a powerful approach to elucidate the regulatory networks of hormone metabolism. The methods involved in the use of liquid chromatography–electrospray ionization–tandem mass spectrometry to develop a high-sensitive and high-throughput hormonome platform are described in this chapter.
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Acknowledgments
The hormone analysis platform described in this chapter has been developed by members in Growth Regulation Research Group, RIKEN Plant Science Center: Yuji Kamiya, Shinjiro Yamaguchi, Eiji Nambara, Mitsunori Seo, Hiroyuki Kasahara, Yusuke Jikumaru, Atsushi Hanada, and Yuri Kanno. We thank Dr. Shinjiro Yamaguchi for critical leading of this manuscript. MS is supported in part by the Japan Society for the Promotion of Science Grant-in-Aid for Young Scientists (B) (21770061).
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Seo, M., Jikumaru, Y., Kamiya, Y. (2011). Profiling of Hormones and Related Metabolites in Seed Dormancy and Germination Studies. In: Kermode, A. (eds) Seed Dormancy. Methods in Molecular Biology, vol 773. Humana Press. https://doi.org/10.1007/978-1-61779-231-1_7
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DOI: https://doi.org/10.1007/978-1-61779-231-1_7
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