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Embryonic Stem Cell Derivation from Human Embryos

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Human Pluripotent Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 767))

Abstract

Human embryonic stem cells (hESCs) are self-renewing, pluripotent cells that serve as a valuable research tool and hold promise for use in regenerative medicine. Most hESC lines are derived from cryopreserved human embryos that were created during in vitro fertilization (IVF) and are in excess of clinical need. Embryos that are discarded during the IVF procedure are also a viable source of hESCs. hESCs can be derived from pre-blastocyst embryos and even from single blastomeres. However, hESC line derivation efficiency is greatest using embryos that have reached the blastocyst stage. This chapter describes a protocol for the derivation of pluripotent stem cell lines from human embryos.

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References

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Author information

Authors and Affiliations

  1. Department of Newborn Medicine, Brigham & Women’s Hospital/Harvard Medical School, Harvard Institutes of Medicine, Boston, MA, USA

    Paul Lerou

Authors
  1. Paul Lerou
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Corresponding author

Correspondence to Paul Lerou .

Editor information

Editors and Affiliations

  1. Research Institute, CHOC Human Neural Stem Cell Resource, Children's Hospital of Orange County, S. Main Street 455, Orange, 92868-3874, California, USA

    Philip H. Schwartz

  2. Beckman Research Institute, Center for Applied Technology Developmen, City of Hope, East Duarte Road 1500, Duarte, 91010, California, USA

    Robin L. Wesselschmidt

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© 2011 Springer Science+Business Media, LLC

About this protocol

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Lerou, P. (2011). Embryonic Stem Cell Derivation from Human Embryos. In: Schwartz, P., Wesselschmidt, R. (eds) Human Pluripotent Stem Cells. Methods in Molecular Biology, vol 767. Humana Press. https://doi.org/10.1007/978-1-61779-201-4_3

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  • DOI: https://doi.org/10.1007/978-1-61779-201-4_3

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-200-7

  • Online ISBN: 978-1-61779-201-4

  • eBook Packages: Springer Protocols

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