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Human Pluripotent Stem Cells pp 221–230Cite as

Flow Cytometric Analysis of Human Pluripotent Stem Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 767))

Abstract

Human pluripotent stem cells, human embryonic stem cells and induced pluripotent stem cells, represent an exciting new era in regenerative medicine and drug discovery. However, prior to their clinical translation, there is a need to gain an in-depth understanding of human pluripotent stem cell biology by characterizing these potentially heterogeneous populations of cells. Flow cytometry provides a rapid and efficient approach with which to isolate, purify, and study the functional properties of defined pluripotent stem cell types.

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Acknowledgments

Methods described in this chapter were developed using funding from the Australian Stem Cell Centre (ASCC) and the NSW/VIC Government Stem Cell Research Grant Program 2008 to ALL. The authors would like to thank James Thomson, University of Wisconsin for the provision of the human iPSC lines, the Australian Stem Cell Centre’s Core hESC Laboratories (Stem Core) for providing cell culture and support services as well as the Flow Core (a collaborative initiative between Monash University, the ASCC, and the Australian Regenerative Medicine Institute (ARMI)) for technical assistance and advice.

Author information

Authors and Affiliations

  1. Department of Anatomy and Developmental Biology, Monash University, Clayton, Australia

    Mirabelle S. H. Ho & Andrew L. Laslett

  2. Flow Core, Australian Stem Cell Centre and Monash University, Clayton, Australia

    Andrew Fryga

Authors
  1. Mirabelle S. H. Ho
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  2. Andrew Fryga
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  3. Andrew L. Laslett
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Corresponding author

Correspondence to Andrew L. Laslett .

Editor information

Editors and Affiliations

  1. Research Institute, CHOC Human Neural Stem Cell Resource, Children's Hospital of Orange County, S. Main Street 455, Orange, 92868-3874, California, USA

    Philip H. Schwartz

  2. Beckman Research Institute, Center for Applied Technology Developmen, City of Hope, East Duarte Road 1500, Duarte, 91010, California, USA

    Robin L. Wesselschmidt

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Ho, M.S.H., Fryga, A., Laslett, A.L. (2011). Flow Cytometric Analysis of Human Pluripotent Stem Cells. In: Schwartz, P., Wesselschmidt, R. (eds) Human Pluripotent Stem Cells. Methods in Molecular Biology, vol 767. Humana Press. https://doi.org/10.1007/978-1-61779-201-4_16

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  • DOI: https://doi.org/10.1007/978-1-61779-201-4_16

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-200-7

  • Online ISBN: 978-1-61779-201-4

  • eBook Packages: Springer Protocols

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