Mechanism of Mo-Dependent Nitrogenase

  • Zhi-Yong Yang
  • Karamatullah Danyal
  • Lance C. SeefeldtEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 766)


Nitrogenase is the enzyme responsible for biological reduction of dinitrogen (N2) to ammonia, a form usable for life. Playing a central role in the global biogeochemical nitrogen cycle, this enzyme has been the focus of intensive research for over 60 years. This chapter provides an overview of the features of nitrogenase as a background to the subsequent chapters of this volume that detail the many methods that have been applied in an attempt to gain a deeper understanding of this complex enzyme.

Key words

Nitrogen fixation Fe protein MoFe protein mechanism metalloenzyme MgATP 



The authors acknowledge the long collaboration with the Brian Hoffman and Dennis Dean laboratories in advancing understanding of nitrogenase. Work in the laboratory of the authors is supported by a generous grant from the National Institutes of Health (GM59087).


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Zhi-Yong Yang
    • 1
  • Karamatullah Danyal
    • 1
  • Lance C. Seefeldt
    • 1
    Email author
  1. 1.Department of Chemistry and BiochemistryUtah State UniversityLoganUSA

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