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Historic Overview of Nitrogenase Research

  • Yilin Hu
  • Markus W. RibbeEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 766)

Abstract

The history of nitrogenase research dates all the way back to the 1800s. This chapter provides a brief account of the advances in this particular research area over the past few hundred years, which include such events as the initial discovery of biological nitrogen fixation, the preparation of active cell-free extracts, the purification of nitrogenase enzyme, the proposal of the Thorneley–Lowe model, and the report of x-ray crystallographic structures of the component proteins of nitrogenase.

Key words

Biological nitrogen fixation nitrogenase cell-free extracts purification x-ray crystallography 

Notes

Acknowledgments

The authors are supported by the National Institutes of Health grant GM 67626 (M.W.R.) and Herman Frasch Foundation grant 617-HF07 (M.W.R.).

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Department of Molecular Biology and BiochemistryUniversity of CaliforniaIrvineUSA

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