Abstract
Vascular endothelial growth factor (VEGF) is a potent inducer of angiogenesis and vascular leak involved in development, wound healing, tumor growth, macular degeneration, and ischemia. Studying the effects of VEGF in vitro is not always sufficient to approximate the complex in vivo response that involves multiple cell types within functioning tissues. Treating mice with an intravenous injection of recombinant VEGF produces a rapid and transient biochemical response that is accompanied by a series of ultrastructural changes. Similar events are induced by hypoxia-induced VEGF in the heart following myocardial infarction or by tumor cell-released VEGF during metastasis. Studying how intact blood vessels respond to VEGF will augment the further development of antipermeability strategies to improve disease progression in a number of pathologies.
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Acknowledgments
The methods described here were developed in the lab of David Cheresh formerly at The Scripps Research Institute in La Jolla and now at the University of California San Diego. The author would like to thank Dr. Cheresh as well as past and present members of the Cheresh lab for their contribution to these projects. The ultrastructural work would not have been possible without the technical expertise of Dr. Malcolm Wood at the TSRI Electron Microscopy Core.
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Weis, S.M. (2011). Evaluation of VEGF-Induced Vascular Permeability in Mice. In: Turksen, K. (eds) Permeability Barrier. Methods in Molecular Biology, vol 763. Humana Press. https://doi.org/10.1007/978-1-61779-191-8_27
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DOI: https://doi.org/10.1007/978-1-61779-191-8_27
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