An In Vitro System to Study Sertoli Cell Blood-Testis Barrier Dynamics

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 763)

Abstract

The use of an in vitro system based on primary cultures of Sertoli cells isolated from rat testes has greatly facilitated the study of the blood-testis barrier in recent years. Herein, we summarize the detailed procedures on the isolation of undifferentiated Sertoli cells from 20-day-old rat testes, the culture of these cells as a monolayer on Matrigel-coated bicameral units, the characterization of these cultured cells, and the use of the Sertoli cell epithelium for monitoring the integrity of the Sertoli cell blood-testis barrier. This information is based on the routine use of this system in our laboratory to study the Sertoli cell blood-testis barrier in the past two decades, which should be helpful for investigators in the field.

Key words

Testis Sertoli cell Blood-testis barrier Tight junction Ectoplasmic specialization Anchoring junction Spermatogenesis 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Center for Biomedical Research, Population CouncilNew YorkUSA

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