Abstract
During malignant transformation, cells can increase their ploidy and hence become polyploid (mostly tetraploid). Frequently, however, tetraploid cells undergo asymmetric divisions, in turn entailing a reduction in ploidy and the acquisition of a pseudo-diploid, aneuploid state. To investigate such a stepwise aneuploidization process, we developed a cytofluorometric method (based on the heterogeneity in cell size and/or chromatin content) that allows for the cloning and subsequent functional analysis of cells with distinct ploidies. Here, we detail this methodology, which has been instrumental for investigating the functional link between ploidy status and oncogenesis.
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Acknowledgments
GK is supported by the Ligue Nationale contre le Cancer (Equipe labellisée), Agence Nationale de la Recherche (ANR), Cancéropôle Ile-de-France, Fondation pour la Recherche Médicale (FRM), Institut National du Cancer (INCa), European Commission (Active p53, Apo-Sys, RIGHT, TransDeath, ChemoRes, ApopTrain), and Fondation pour la Recherche Médicale. MC is funded by the Association pour la recherche sur le cancer (ARC). LG, IV, and LS are supported by Apo-Sys, La Ligue contre le Cancer and FRM, respectively.
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Castedo, M. et al. (2011). Cytofluorometric Purification of Diploid and Tetraploid Cancer Cells. In: Banfalvi, G. (eds) Cell Cycle Synchronization. Methods in Molecular Biology, vol 761. Humana Press. https://doi.org/10.1007/978-1-61779-182-6_3
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DOI: https://doi.org/10.1007/978-1-61779-182-6_3
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