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Synchronization of In Vitro Maturation in Porcine Oocytes

  • Tamas SomfaiEmail author
  • Yuji Hirao
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 761)

Abstract

When removed from the follicles, during the 44 h process of in vitro maturation (IVM) fully grown porcine oocytes resume meiosis spontaneously from the late diplotene stage of the first meiotic prophase and proceed to the metaphase-II (MII) stage at which they remain arrested until fertilization. However, the resumption may start at various times causing heterogeneity in the nuclear stage and also in cytoplasmic characteristics (i.e., the activity of certain protein kinases) within a population. Those oocytes that reach the MII stage earlier than others undergo an ageing process which is detrimental for further embryo development. The synchronization of nuclear progression is possible by a transient inhibition of meiotic resumption during the first 20–22 h of IVM either by (1) the elevation of intracellular levels of cyclic adenosine monophosphate (cAMP) or (2) suppressing the activity of the metaphase promoting factor (MPF). A protocol for each approach is described.

Key words

Porcine oocyte in vitro maturation cAMP MPF 

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© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.National Agriculture and Food Research Organization (NARO), National Institute of Livestock and Grassland Science (NILGS)IbarakiJapan
  2. 2.National Agriculture and Food Research Organization (NARO), National Agricultural Research Center for Tohoku Region (NARCT)MoriokaJapan

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