Identifying G Protein-Coupled Receptor Escorts, Chaperones, and Intracellular Tethers Regulating Receptor Density at the Cell Surface

  • Stefano MarulloEmail author
  • Liliana Pardo Lopez
  • Lamia Achour
Part of the Neuromethods book series (NM, volume 60)


G protein-coupled receptor (GPCR) responsiveness is dynamically regulated by various mechanisms, allowing fine-tuning of cell signaling. Modulation of GPCR plasma membrane density, via their release from intracellular compartments, constitutes a recently identified important process in this context. This phenomenon requires a complex network of interactions between GPCRs, “private” chaperones and escort proteins, and gatekeepers, which are directly involved in the retention of GPCRs in the intracellular compartments. The molecular and functional characterization of the players in this game is at its very beginning and requires appropriate quantitative methods of investigation to unravel the mechanisms that are involved.

Key words

GPCR Chaperone Escort protein Secretory pathway Resensitization 



The authors thank Drs. Mark GH Scott and Hervé Enslen for the critical reading of the manuscript. This work is supported by a grant (#R09158KK) of the Agence Nationale de la Recherche sur le SIDA (ANRS) to SM. The sabbatical leave of LPL was supported by a grant from CONACyT.


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Stefano Marullo
    • 1
    Email author
  • Liliana Pardo Lopez
  • Lamia Achour
  1. 1.AchourInstitut Cochin,Université Paris Descartes, CNRS-INSERMParisFranceL

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