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Methods for the Discovery and Characterization of G Protein-Coupled Receptors pp 167–185Cite as

Identifying G Protein-Coupled Receptor Escorts, Chaperones, and Intracellular Tethers Regulating Receptor Density at the Cell Surface

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Part of the book series: Neuromethods ((NM,volume 60))

Abstract

G protein-coupled receptor (GPCR) responsiveness is dynamically regulated by various mechanisms, allowing fine-tuning of cell signaling. Modulation of GPCR plasma membrane density, via their release from intracellular compartments, constitutes a recently identified important process in this context. This phenomenon requires a complex network of interactions between GPCRs, “private” chaperones and escort proteins, and gatekeepers, which are directly involved in the retention of GPCRs in the intracellular compartments. The molecular and functional characterization of the players in this game is at its very beginning and requires appropriate quantitative methods of investigation to unravel the mechanisms that are involved.

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Acknowledgements

The authors thank Drs. Mark GH Scott and Hervé Enslen for the critical reading of the manuscript. This work is supported by a grant (#R09158KK) of the Agence Nationale de la Recherche sur le SIDA (ANRS) to SM. The sabbatical leave of LPL was supported by a grant from CONACyT.

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  1. AchourInstitut Cochin,, Université Paris Descartes, CNRS-INSERM, Paris, FranceL

    Stefano Marullo

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  1. Stefano Marullo
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  2. Liliana Pardo Lopez
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  3. Lamia Achour
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Correspondence to Stefano Marullo .

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  1. Center for Health Sciences, College of Osteopathic Medicine, Oklahoma State University, W. 17th Street 1111, Tulsa, 74107, Oklahoma, USA

    Craig W. Stevens

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Marullo, S., Lopez, L.P., Achour, L. (2011). Identifying G Protein-Coupled Receptor Escorts, Chaperones, and Intracellular Tethers Regulating Receptor Density at the Cell Surface. In: Stevens, C. (eds) Methods for the Discovery and Characterization of G Protein-Coupled Receptors. Neuromethods, vol 60. Humana Press. https://doi.org/10.1007/978-1-61779-179-6_9

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  • DOI: https://doi.org/10.1007/978-1-61779-179-6_9

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