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Using RNA Interference to Downregulate G Protein-Coupled Receptors

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Book cover Methods for the Discovery and Characterization of G Protein-Coupled Receptors

Part of the book series: Neuromethods ((NM,volume 60))

Abstract

Technologies developed to interfere with gene transcripts were developed back in the 1980. However, it was not before the last decade that light was shed on the underlying mechanisms of what is now known as RNA interference. From then, RNAi was propelled to the forefront as a revolutionizing approach for basic research and clinical therapy. In the present chapter, we will present an overview of RNAi and its mechanisms with a focus on GPCRs applied to neuroscience. We will briefly detail the steps to move from the in vitro assessment to the in vivo proof-of-principle to further ensure appropriate clinical transfer. Examples of successful experiments will be given in each section. Advances, drawbacks, and future directions will be discussed with RNAi as an exciting new technology that can be used to treat GPCR-related neuropathologies intractable with commonly-used pharmacological agents.

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Authors and Affiliations

  1. Department of Physiology and Biophysics, Centre des Neurosciences de Sherbrooke, Université de Sherbrooke, Sherbrooke, QC, Canada

    Nicolas Beaudet

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  1. Philippe Sarret
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  2. Louis Doré-Savard
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  3. Pascal Tétreault
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  4. Valérie Bégin-Lavallée
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  5. Marc-André Dansereau
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  6. Nicolas Beaudet
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Correspondence to Nicolas Beaudet .

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Editors and Affiliations

  1. Center for Health Sciences, College of Osteopathic Medicine, Oklahoma State University, W. 17th Street 1111, Tulsa, 74107, Oklahoma, USA

    Craig W. Stevens

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Sarret, P., Doré-Savard, L., Tétreault, P., Bégin-Lavallée, V., Dansereau, MA., Beaudet, N. (2011). Using RNA Interference to Downregulate G Protein-Coupled Receptors. In: Stevens, C. (eds) Methods for the Discovery and Characterization of G Protein-Coupled Receptors. Neuromethods, vol 60. Humana Press. https://doi.org/10.1007/978-1-61779-179-6_19

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  • DOI: https://doi.org/10.1007/978-1-61779-179-6_19

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