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High-Throughput Analyses and Curation of Protein Interactions in Yeast

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Yeast Systems Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 759))

Abstract

The yeast Saccharomyces cerevisiae is the model organism in which protein interactions have been most extensively analyzed. The vast majority of these interactions have been characterized by a variety of sophisticated high-throughput techniques probing different aspects of protein association. This chapter summarizes the major techniques, highlights their complementary nature, discusses the data they produce, and highlights some of the biases from which they suffer. A main focus is the key role played by computational methods for processing, analyzing, and validating the large body of noisy data produced by the experimental procedures. It also describes how computational methods are used to extend the coverage and reliability of protein interaction data by integrating information from heterogeneous sources and reviews the current status of literature-curated data on yeast protein interactions stored in specialized databases.

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Acknowledgments

The authors thank Brian Turner and Andrei Turinsky for their work on the consolidation of protein interaction data and for help with iRefWeb. Funding is acknowledged from the Canadian Institutes of Health Research (CIHR MOP#82940), the Ontario Research Fund, and the SickKids Foundation. SJW is Canada Research Chair, Tier 1.

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Authors and Affiliations

  1. Molecular Structure and Function Program, Hospital for Sick Children, Toronto, ON, Canada

    Shoshana J. Wodak, Jim Vlasblom & Shuye Pu

  2. Department of Biochemistry, University of Toronto, Toronto, ON, Canada

    Shoshana J. Wodak & Jim Vlasblom

  3. Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada

    Shoshana J. Wodak

Authors
  1. Shoshana J. Wodak
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  2. Jim Vlasblom
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  3. Shuye Pu
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Correspondence to Shoshana J. Wodak .

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Editors and Affiliations

  1. , Department of Biochemistry, University of Cambridge, Tennis Court Road 80, Cambridge, CB2 1GA, United Kingdom

    Juan I. Castrillo

  2. , Department of Biochemistry, University of Cambridge, Tennis Court Road 80, Cambridge, CB2 1GA, United Kingdom

    Stephen G. Oliver

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Wodak, S.J., Vlasblom, J., Pu, S. (2011). High-Throughput Analyses and Curation of Protein Interactions in Yeast. In: Castrillo, J., Oliver, S. (eds) Yeast Systems Biology. Methods in Molecular Biology, vol 759. Humana Press. https://doi.org/10.1007/978-1-61779-173-4_22

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  • DOI: https://doi.org/10.1007/978-1-61779-173-4_22

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