Abstract
Integrin LFA-1 plays an important role in leukocyte trafficking to inflammatory sites as well as the interaction of T cells with antigen-presenting cells. Inhibition of the LFA-1:ICAM-1 interactions is a promising therapeutic approach for alleviating inflammation and autoimmune diseases. This chapter describes cell-free assays to study the LFA-1:ICAM-1 interactions. These assays may be used for screening novel LFA-1 antagonists.
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The author would like to thank Dr. Motomu Shimaoka for supporting the research.
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Yuki, K. (2011). Cell-Free Ligand-Binding Assays for Integrin LFA-1. In: Shimaoka, M. (eds) Integrin and Cell Adhesion Molecules. Methods in Molecular Biology, vol 757. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-166-6_6
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DOI: https://doi.org/10.1007/978-1-61779-166-6_6
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