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MicroRNA Profiling Using RNA from Microdissected Immunostained Tissue

  • Clemens L. Bockmeyer
  • Danny Jonigk
  • Hans Kreipe
  • Ulrich LehmannEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 755)

Abstract

mRNA expression profiling has been used to define molecular subtypes of human breast cancer. Also microRNAs have been investigated in these breast cancer subtypes. However, little is known regarding the microRNA signature of healthy luminal and basal breast epithelial cells. Therefore, a method is described to isolate immunostained luminal and basal breast epithelial cells in formalin-fixed paraffin-embedded tissues by laser microdissection. Employing this new methodological approach, we could identify distinct microRNA profiles of luminal and basal breast epithelial cells by real-time PCR-based profiling.

Key words

Breast Laser microdissection miRNA Myoepithelial Luminal 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Clemens L. Bockmeyer
    • 1
  • Danny Jonigk
    • 1
  • Hans Kreipe
    • 1
  • Ulrich Lehmann
    • 1
    Email author
  1. 1.Institute of PathologyMedizinische Hochschule HannoverHannoverGermany

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