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Quantitative RT-PCR Gene Expression Analysis of a Laser Microdissected Placenta: An Approach to Study Preeclampsia

  • Yuditiya Purwosunu
  • Akihiko SekizawaEmail author
  • Takashi Okai
  • Tetsuhiko Tachikawa
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 755)

Abstract

Preeclampsia is still one of the leading causes of maternal and neonatal mortality and morbidity. Despite intensive research, the cause of preeclampsia has not yet been established. However, for a variety of reasons, the numerous aspects of normal and pathological pregnancies, particularly with regard to preeclampsia, remain difficult to study and are, therefore, poorly understood. The development of a laser-based microdissection system has provided rapid morphologically and phenotypically distinct types of cells in the placenta for molecular analysis. Alterations in gene expression in the cytotrophoblast and syncytiotrophoblast or other specific placental cell types from patients who later develop preeclampsia have been reported. Laser microdissection is an attractive method to study each specific placental cell type to characterize the development of preeclampsia or to study the pathophysiology of preeclampsia. This method may contribute to a better understanding of the pathophysiology of preeclampsia.

Key words

Preeclampsia Laser microdissection Placenta RT-PCR 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Yuditiya Purwosunu
    • 1
    • 2
  • Akihiko Sekizawa
    • 3
    Email author
  • Takashi Okai
    • 1
  • Tetsuhiko Tachikawa
    • 4
  1. 1.Department of Obstetrics and GynecologyShowa University School of MedicineTokyoJapan
  2. 2.Department of Obstetrics and GynecologyUniversity of Indonesia, Cipto Mangunkusumo National HospitalJakartaIndonesia
  3. 3.Department of Obstetrics and GynecologyShowa University School of MedicineTokyoJapan
  4. 4.Department of Oral PathologyShowa University School of DentistryTokyoJapan

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