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Immunoguided Microdissection Techniques

  • Michael A. Tangrea
  • Jeffrey C. Hanson
  • Robert F. Bonner
  • Thomas J. Pohida
  • Jaime Rodriguez-Canales
  • Michael R. Emmert-BuckEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 755)

Abstract

Over the past 15 years, laser-based microdissection has improved the precision by which scientists can procure cells of interest from a heterogeneous tissue section. However, for studies that require a large amount of material (e.g., proteomics) or for cells that are scattered and difficult to identify by standard histological stains, an immunostain-based, automated approach becomes essential. In this chapter, we discuss the use of immunohistochemistry (IHC) and immunofluorescence (IF) to guide the microdissection process via manual and software-driven auto-dissection methods. Although technical challenges still exist with these innovative approaches, we present here methods and protocols to successfully perform immuno-based microdissection on commercially available laser dissection systems.

Key words

Automation Immunohistochemistry Microdissection 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Michael A. Tangrea
    • 2
  • Jeffrey C. Hanson
    • 2
  • Robert F. Bonner
    • 2
  • Thomas J. Pohida
    • 2
  • Jaime Rodriguez-Canales
    • 2
  • Michael R. Emmert-Buck
    • 1
    Email author
  1. 1.Pathogenetics Unit and Laser Microdissection Core, Laboratory of PathologyCenter for Cancer ResearchGaithersburgUSA
  2. 2.Pathogenetics Unit and Laser Microdissection Core, Laboratory of PathologyCenter for Cancer Research, National Cancer Institute, National Institutes of HealthBethesdaUSA

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