Laser-Capture Microdissection for Factor VIII-Expressing Endothelial Cells in Cancer Tissues

  • Tomoatsu Kaneko
  • Takashi Okiji
  • Reika Kaneko
  • Hideaki Suda
  • Jacques E. Nör
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 755)

Abstract

The immunostaining based Laser-capture microdissection (LCM) method called immune-LCM allows us to quantify the mRNA. Immune-LCM has recently been introduced to enhance identification of cells carrying a particular protein from frozen tissue samples. We have recently performed the immune-LCM of formaldehyde-fixated, paraffin-embedded tissues immunostained with a monoclonal antibody Factor VIII. This method could be useful for quantitative gene expression analysis in blood vessels from tumors of patients that have been treated with antiangiogenic drugs, allowing for validation of the effect of drug on the expected targets. Such capability might be exceedingly useful for the evaluation of the bioactivity of new drugs. This method is also useful to compare gene expression patterns in tumor cells versus endothelial cells during tumor progression or tumor angiogenesis.

Key words

Laser-capture microdissection Endothelial cells Gene expression Immune-LC FFPE tissues 

References

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Tomoatsu Kaneko
    • 1
  • Takashi Okiji
    • 1
  • Reika Kaneko
    • 2
  • Hideaki Suda
    • 3
  • Jacques E. Nör
    • 4
    • 5
    • 6
  1. 1.Cariology, Operative Dentistry and EndodonticsNiigata University Graduate School of Medical and Dental SciencesNiigataJapan
  2. 2.Applied Molecular MedicineNiigata University Graduate School of Medical and Dental SciencesChuo-KuJapan
  3. 3.Pulp Biology and Endodontics, Department of Restorative Sciences, Graduate School of Medical and Dental SciencesTokyo Medical and Dental UniversityBunkyo-kuJapan
  4. 4.Cariology, Restorative Sciences, and Endodontics, School of DentistryUniversity of MichiganAnn ArborUSA
  5. 5.Department of Biomedical Engineering, College of EngineeringUniversity of MichiganAnn ArborUSA
  6. 6.Comprehensive Cancer CenterUniversity of MichiganAnn ArborUSA

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