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Gene Expression Using the PALM System

  • Jian-Xin Lu
  • Cheuk-Chun SzetoEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 755)

Abstract

The PALM Robot MicroBeam laser microdissection system can isolate specified cells from complex tissues section, in a rapid and precise manner. Combined with other methods, PALM may be used for gene expression elucidating the role of specialized cell type in physiological and pathological activity. This chapter describes the application of the PALM MicroBeam system to isolate RNA from cells in a complex tissue for subsequent gene expression analysis. Protocols show the steps from preparation of tissue samples to the final quantitative results. The process is articulated in several steps, each of which requires optimal choices in order to obtain reliable data from a limited number of cells (500–10,000 cells). Furthermore, the notes regarding tissue preparation, microdissection of the interested cells, are also emphasized.

Key words

Gene expression PALM Microdissection RNA Real-time quantitative PCR 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Department of Medicine & Therapeutics, Prince of Wales HospitalThe Chinese University of Hong KongHong KongChina

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