Application of Laser-Capture Microdissection to Study Renal Carcinogenesis

  • Kerstin Stemmer
  • Daniel R. DietrichEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 755)


Kidney cancer is characterized by significant morphological and molecular heterogeneity. Evaluation of mechanisms involved in the development and progression of kidney cancer require comprehensive analyses of genomes, transcriptomes, proteomes, and methylation profiles in normal and tumor tissue. To date, indiscriminate homogenates of tumor tissue or biopsy samples have been used as a source for DNA, RNA, or protein isolation. A major technical improvement has been the development of laser-assisted microdissection that allows the isolation of morphologically similar cells. The applications of this techno­logy to kidney cancer research are outlined.

Key words

Kidney Kidney cancer Microarray RNA Renal carcinogenesis Transcriptional profiling 


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Human and Environmental ToxicologyUniversity of KonstanzKonstanzGermany
  2. 2.Department of Internal Medicine, Metabolic Diseases InstituteUniversity of CincinnatiCincinnatiUSA

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