Application of Laser-Capture Microdissection to Study Renal Carcinogenesis
Kidney cancer is characterized by significant morphological and molecular heterogeneity. Evaluation of mechanisms involved in the development and progression of kidney cancer require comprehensive analyses of genomes, transcriptomes, proteomes, and methylation profiles in normal and tumor tissue. To date, indiscriminate homogenates of tumor tissue or biopsy samples have been used as a source for DNA, RNA, or protein isolation. A major technical improvement has been the development of laser-assisted microdissection that allows the isolation of morphologically similar cells. The applications of this technology to kidney cancer research are outlined.
Key wordsKidney Kidney cancer Microarray RNA Renal carcinogenesis Transcriptional profiling
- 1.Eble, J., Sauter, G., Epstein, J., and Sesterhenn, I. (2004) Pathology and Genetics of Tumors of the Urinary System and Male Genital Organs, IARC Press, WHO Classification of Tumours, Volume 7.Google Scholar
- 4.Zhu, G., Reynolds, L., Crnogorac-Jurcevic, T., Gillett, C. E., Dublin, E. A., Marshall, J. F., Barnes, D., D’Arrigo, C., Van Trappen, P. O., Lemoine, N. R., and Hart, I. R. (2003) Combination of microdissection and microarray analysis to identify gene expression changes between differentially located tumour cells in breast cancer, Oncogene 22, 3742–3748.CrossRefGoogle Scholar
- 5.Castro, N. P., Osorio, C. A., Torres, C., Bastos, E. P., Mourao-Neto, M., Soares, F. A., Brentani, H. P., and Carraro, D. M. (2008) Evidence that molecular changes in cells occur before morphological alterations during the progression of breast ductal carcinoma, Breast Cancer Res 10, R87.CrossRefGoogle Scholar
- 9.Dietrich, D. R., Heussner, A. H., O’Brien, E., Gramatte, T., Runkel, M., Rumpf, S., and Day, B. W. (2008) Propiverine-induced accumulation of nuclear and cytosolic protein in F344 rat kidneys: isolation and identification of the accumulating protein, Toxicol Appl Pharmacol 233, 411–419.CrossRefGoogle Scholar