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Oligonucleotide Microarray Expression Profiling of Contrasting Invasive Phenotypes in Colorectal Cancer

  • Christopher C. ThornEmail author
  • Deborah Williams
  • Thomas C. Freeman
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 755)

Abstract

This chapter refers to the application of laser-capture microdissection with oligonucleotide microarray analysis. The protocol described has been successfully used to identify differential transcript expression between contrasting colorectal cancer invasive phenotypes. Tissue processing, RNA extraction, quality control, amplification, fluorescent labelling, purification, hybridisation, and elements of data analysis are covered.

Key words

Oligonucleotide microarray Laser-capture microdissection Invasion Colorectal 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Christopher C. Thorn
    • 1
    Email author
  • Deborah Williams
    • 2
  • Thomas C. Freeman
    • 3
  1. 1.Department of Academic SurgerySt. James’s University HospitalLeedsUK
  2. 2.MRC HarwellOxfordUK
  3. 3.Roslin InstituteUniversity of EdinburghEdinburghUK

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