Synthesis of Long DNA-Based Nanowires
Here we describe novel procedures for production of DNA-based nanowires. This include synthesis and characterization of the one-to-one double-helical complex of poly(dG)–poly(dC), triple-helical poly(dG)–poly(dG)–poly(dC) and G4-DNA, which is a quadruple-helical form of DNA. All these types of DNA-based molecules were synthesized enzymatically using Klenow exo− fragment of DNA Polymerase I. All the above types of nanowires are characterized by a narrow-size distribution of molecules. The contour length of the molecules can be varied from tens to hundreds of nanometers. These structures possess improved conductive and mechanical properties with respect to a canonical random-sequenced DNA and can possibly be used as wire-like conducting or semiconducting nanostructures in the field of nanoelectronics.
Key wordsDNA nanowires Enzymatic synthesis Klenow exo− Poly(dG)–poly(dC) G4-DNA Triplex DNA
This work was supported by the EC through the contracts IST-2001-38951 (“DNA-Based Nanowires”) and FP6-029192 (“DNA-Based Nanodevices”).
- 7.Kotlyar, A. B., Borovok, N., Molotsky T., Fadeev L., and Gozin M. (2005) In Vitro synthesis of uniform Poly(dG)-Poly(dC) by Klenow exo– fragment of Polymerase I. Nucl. Acid Res. 33, 525–535.Google Scholar
- 12.Sun, J. S., Garestier, T. and Helene, C. (1996) Oligonucleotide directed triple helix formation. Curr. Opin. Struct. Biol., 6, 327–333.Google Scholar
- 14.Kotlyar, A. B., Borovok, N., Molotsky, T, Klinov, D., Dwir, B. and Kapon E. Synthesis of novel poly(dG)-poly(dG)-poly(dC) triplex structure by Klenow exo- fragment of DNA polymerase I. 2005 Nucl. Acid Res. 33, 6515–6521.Google Scholar
- 23.Borovok, N, Molotsky, T, Ghabboun, J, Porath, D. and Kotlyar, A. (2008) Efficient procedure of preparation and properties of long uniform G4-DNA nanowires. Anal. Biochem. 374, 71–78.Google Scholar