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DNA Nanotechnology

Volume 749 of the series Methods in Molecular Biology pp 105-113

Date:

Preparation and Atomic Force Microscopy of Quadruplex DNA

  • James VesenkaAffiliated withDepartment of Chemistry and Physics, University of New England Email author 

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Abstract

The purpose of this chapter is to provide detailed instructions for the preparation and atomic force microscopy (AFM) imaging of linear chains of quadruplex DNA (a.k.a. “G-wire DNA”). Successful self-assembly of long chain quadruplex DNA requires pure concentrated guanine-rich oligonucleotide sequence (GROs) and monovalent cations in a growth buffer. AFM imaging of individual G-wire DNA strands requires many carefully monitored steps, including substrate preparation, G-wire concentration, adsorption onto substrate, rinsing, drying, appropriate selection/use of imaging probes, and dry atmosphere imaging conditions. Detailed step-wise instructions are provided.

Key words

G-wire Quadruplex DNA Guanine-rich oligonucleotide Self-assembly Atomic force microscopy