Preparation and Atomic Force Microscopy of Quadruplex DNA

  • James VesenkaEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 749)


The purpose of this chapter is to provide detailed instructions for the preparation and atomic force microscopy (AFM) imaging of linear chains of quadruplex DNA (a.k.a. “G-wire DNA”). Successful self-assembly of long chain quadruplex DNA requires pure concentrated guanine-rich oligonucleotide sequence (GROs) and monovalent cations in a growth buffer. AFM imaging of individual G-wire DNA strands requires many carefully monitored steps, including substrate preparation, G-wire concentration, adsorption onto substrate, rinsing, drying, appropriate selection/use of imaging probes, and dry atmosphere imaging conditions. Detailed step-wise instructions are provided.

Key words

G-wire Quadruplex DNA Guanine-rich oligonucleotide Self-assembly Atomic force microscopy 


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Department of Chemistry and PhysicsUniversity of New EnglandBiddefordUSA

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