Protocol

DNA Nanotechnology

Volume 749 of the series Methods in Molecular Biology pp 93-104

Date:

G-Quartet, G-Quadruplex, and G-Wire Regulated by Chemical Stimuli

  • Daisuke Miyoshi
  • , Naoki SugimotoAffiliated withFaculty of Frontiers of Innovative Research in Science and Technology (FIRST), and Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University Email author 

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Abstract

Guanine-rich DNA, which is widely distributed in the human genome, can fold into a supramolecular structure called the G-wire. The G-wire possesses promising characteristics as a functional element for various applications in vitro and in vivo. Here, we describe the preparative procedures for the G-wire and signatures of G-wire formation. Procedures for the regulation of G-wire formation by chemical stimuli will be useful for in vivo and in vitro applications.

Key words

G-wire G-quadruplex G-quartet Guanine Molecular crowding Metal ion Polymorphism