Abstract
Artificial DNA nanostructures such as DNA origami have garnered significant interest as templates for sub-20 nm lithography because their rational design allows for the incorporation of binding sites to assemble nanocomponents with 6 nm resolution. In addition, their overall size of 100 nm is easily accessible by top-down lithographic methods. Combining the strengths of top-down lithography and bottom-up self-assembly using DNA nanostructures may provide a commercially viable route to fabricating electronic and photonic devices with nanometer-scale features. We have demonstrated just such a comprehensive process in which 5 nm gold nanoparticles are first assembled in high yield on DNA origami. The constructs are then organized, rinsed, and dried on patterned silicon substrates, yielding large area arrays of both origami and nanoparticles.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Seeman, N. C. (2003) DNA in a material world Nature 421, 427–431.
Whitesides, G. M., Grzybowski, B. (2002) Self-assembly at all scales Science 295, 2418–2421.
Rothemund, P. W. K. (2006) Folding DNA to create nanoscale shapes and patterns Nature 440, 297–302.
Aldaye, F. A., Palmer, A. L., Sleiman, H. F. (2008) Assembling materials with DNA as the guide Science 321, 1795–1799.
Lin, C. X., Liu, Y., Rinker, S., Yan, H. (2006) DNA tile based self-assembly: Building complex nanoarchitectures Chemphyschem 7, 1641–1647.
Hung, A. M., Micheel, C. M., Bozano, L. D., Osterbur, L. W., Wallraff, G. M., Cha, J. N. (2010) Large area spatially ordered arrays of gold nanoparticles directed by lithographically confined DNA origami Nature NanoÂtechnology, 5, 121–126.
Pastre, D., Hamon, L., Landousy, F., Sorel, I., David, M. O., Zozime, A., Le Cam, E., Pietrement, O. (2006) Anionic polyelectrolyte adsorption on mica mediated by multivalent cations: A solution to DNA imaging by atomic force microscopy under high ionic strengths Langmuir 22, 6651–6660.
Kershner, R. J., Bozano, L. D., Micheel, C. M., Hung, A. M., Fornof, A. R., Cha, J. N., Rettner, C. T., Bersani, M., Frommer, J., Rothemund, P. W. K., Wallraff, G. M. (2009) Placement and orientation of individual DNA shapes on lithographically patterned surfaces Nature Nanotechnology 4, 557–561.
Zanchet, D., Micheel, C. M., Parak, W. J., Gerion, D., Alivisatos, A. P. (2001) Electrophoretic isolation of discrete Au nanocrystal/DNA conjugates Nano Letters 1, 32–35.
Demers, L. M., Mirkin, C. A., Mucic, R. C., Reynolds, R. A., Letsinger, R. L., Elghanian, R., Viswanadham, G. (2000) A fluorescence-based method for determining the surface coverage and hybridization efficiency of thiol-capped oligonucleotides bound to gold thin films and nanoparticles Analytical Chemistry 72, 5535–5541.
Hansma, H. G., Bezanilla, M., Zenhausern, F., Adrian, M., Sinsheimer, R. L. (1993) Atomic Force Microscopy of DNA in Aqueous-Solutions Nucleic Acids Research 21, 505–512.
Acknowledgments
The authors thank Luisa Bozano for providing the lithographically patterned substrates and Christine M. Micheel for helping to develop the methods for binding nanoparticles to DNA origami. This work was financially supported by the Center on Polymer Interfaces and Macromolecular Assemblies (Award Number: NSF DMR 0213618), the Office of Naval Research (Award Number: N00014-09-01-0250), and UCSD startup funds.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2011 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Hung, A.M., Cha, J.N. (2011). Templated Assembly of DNA Origami Gold Nanoparticle Arrays on Lithographically Patterned Surfaces. In: Zuccheri, G., Samorì, B. (eds) DNA Nanotechnology. Methods in Molecular Biology, vol 749. Humana Press. https://doi.org/10.1007/978-1-61779-142-0_13
Download citation
DOI: https://doi.org/10.1007/978-1-61779-142-0_13
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-61779-141-3
Online ISBN: 978-1-61779-142-0
eBook Packages: Springer Protocols