Templated Assembly of DNA Origami Gold Nanoparticle Arrays on Lithographically Patterned Surfaces

Part of the Methods in Molecular Biology book series (MIMB, volume 749)


Artificial DNA nanostructures such as DNA origami have garnered significant interest as templates for sub-20 nm lithography because their rational design allows for the incorporation of binding sites to assemble nanocomponents with 6 nm resolution. In addition, their overall size of 100 nm is easily accessible by top-down lithographic methods. Combining the strengths of top-down lithography and bottom-up self-assembly using DNA nanostructures may provide a commercially viable route to fabricating electronic and photonic devices with nanometer-scale features. We have demonstrated just such a comprehensive process in which 5 nm gold nanoparticles are first assembled in high yield on DNA origami. The constructs are then organized, rinsed, and dried on patterned silicon substrates, yielding large area arrays of both origami and nanoparticles.

Key words

DNA origami Gold nanoparticles Template-directed self-assembly Lithography 



The authors thank Luisa Bozano for providing the lithographically patterned substrates and Christine M. Micheel for helping to develop the methods for binding nanoparticles to DNA origami. This work was financially supported by the Center on Polymer Interfaces and Macromolecular Assemblies (Award Number: NSF DMR 0213618), the Office of Naval Research (Award Number: N00014-09-01-0250), and UCSD startup funds.


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Department of NanoengineeringUC San DiegoLa JollaUSA

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