Controlled Confinement of DNA at the Nanoscale: Nanofabrication and Surface Bio-Functionalization

  • Matteo Palma
  • Justin J. Abramson
  • Alon A. Gorodetsky
  • Colin Nuckolls
  • Michael P. Sheetz
  • Shalom J. Wind
  • James Hone
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 749)

Abstract

Nanopatterned arrays of biomolecules are a powerful tool to address fundamental issues in many areas of biology. DNA nanoarrays, in particular, are of interest in the study of DNA–protein interactions and for biodiagnostic investigations. In this context, achieving a highly specific nanoscale assembly of oligonucleotides at surfaces is critical. In this chapter, we describe a method to control the immobilization of DNA on nanopatterned surfaces; the nanofabrication and the bio-functionalization involved in the process will be discussed.

Key words

DNA Self-assembly Nanoscale Nanotechnology Fluorescence microscopy 

Notes

Acknowledgments

We gratefully acknowledge support from the office of Naval Research under award number N00014-09-1-1117, National Institutes of Health through award number PN2EY016586 under the NIH Roadmap for Medical Research, and from the National Science Foundation under NSF award number EF-05-07086 and award number CHE-0936923. Additional support from the Nanoscale Science and Engineering Initiative of the National Science Foundation under NSF Award Number CHE-0641523 and from the New York State Office of Science, Technology, and Academic Research (NYSTAR) is also gratefully acknowledged.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Matteo Palma
    • 1
  • Justin J. Abramson
  • Alon A. Gorodetsky
  • Colin Nuckolls
  • Michael P. Sheetz
  • Shalom J. Wind
  • James Hone
  1. 1.Department of Mechanical Engineering & Applied Physics and Applied MathematicsColumbia UniversityNew YorkUSA

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