Abstract
The determination of pesticide residues by HPLC-MS/MS requires decisions on a multitude of analytical parameters. This includes the selection of eluents, columns and ion sources, but also the optimization of the tandem mass spectrometer for the selected target analytes. Another aspect is the use of the restricted acquisition time between two chromatographic data points. An appropriate selection of all these parameters as well as the measures to avoid interference by cross talks and wrong quantitative results by matrix effects is discussed in this chapter.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Tomlin C.D.S. (ed) 2006. The Pesticide Manual - A World Compendium. Fourteenth edition, British Crop Protection Council (BCPC), Hampshire GU34 2QD, UK
Pico Y, Blasco C, Font G. (2004) Environmental and food applications of LC-tandem mass spectrometry in pesticide-residue analysis: an overview. Mass Spectrom. Rev. 23, 45–85
Alder L,. Greulich K, Kempe G, and Vieth B (2006) Residue Analysis of 500 High Priority Pesticides – better by GC-MS or LC-MS/MS? Mass Spectrom. Rev. 25, 838–865
Data Pool of the EU Community Reference Laboratories for Residues of Pesticides: http://www.crl-pesticides-datapool.eu/
Kostiainen, R.; Kauppila, T.J. (2009) Effect of eluent on the ionization process in liquid chromatography-mass spectrometry. J. Chromatogr. A 1216, 685–699
Taylor, P. J. (2005) Matrix effects: the Achilles heel of quantitative high-performance liquid chromatography–electrospray–tandem mass spectrometry. Clinical Biochemistry 38, 328–334
Bruins, A. P. (1998) Mechanistic aspects of electrospray ionization. J. Chromatogr. A 794, 345–357
Enke, C. G. (1997) A predictive model for matrix and analyte effects in electrospray ionization of singly-charged ionic analytes. Anal. Chem., 69, 4885–4893
Cech, N. B.; Enke, C. G. (2001) Practical implications of some recent studies in electrospray ionization fundamentals. Mass Spectrom. Rev. 20, 362–368
King, R.; Bonfiglio, R.; Fernandez-Metzler, C.; Miller-Stein, C.; Olah, T. (2000) Mechanistic investigation of ionization suppression in electrospray ionization. J. Amer. Soc. Mass Spectrom. 11, 942–950
Method validation and quality control procedures for pesticide residue analysis in food and feed; Document N° SANCO/2007/3131; http://ec.europa.eu/food/plant/protection/resources/qualcontrol_en.pdf
Rychlik M and Asam S (2008) Stable isotope dilution assays in mycotoxin analysis. Anal. Bioanal. Chem. 390, 617–628
Benijts T, Dams R, Lambert W, and De Leenheer A (2004) Countering matrix effects in environmental liquid chromatography–electrospray ionization tandem mass spectrometry water analysis for endocrine disrupting chemicals. J. Chromatogr. A 1029, 153–159
Lindegardh N, Annerberg A, White NJ, and Day NPJ (2008) Development and validation of a liquid chromatographic-tandem mass spectrometric method for determination of piperaquine in plasma Stable isotope labeled internal standard does not always compensate for matrix effects. J. Chromatogr. B 862, 227–236
Wang S, Cyronak M, and Yang E (2007) Does a stable isotopically labeled internal standard always correct analyte response?: A matrix effect study on a LC/MS/MS method for the determination of carvedilol enantiomers in human plasma. J. Pharm. Biomed. Anal. 43, 701–707
Stahnke H, Reemtsma T, Alder L (2009) Compensation of Matrix Effects by Postcolumn Infusion of a Monitor Substance in Multiresidue Analysis with LC–MS/MS. Anal. Chem. 81, 2185–2192
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2011 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Alder, L. (2011). Targeted Pesticide Residue Analysis Using Triple Quad LC-MS/MS. In: Zweigenbaum, J. (eds) Mass Spectrometry in Food Safety. Methods in Molecular Biology, vol 747. Humana Press. https://doi.org/10.1007/978-1-61779-136-9_7
Download citation
DOI: https://doi.org/10.1007/978-1-61779-136-9_7
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-61779-135-2
Online ISBN: 978-1-61779-136-9
eBook Packages: Springer Protocols