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Multi Mycotoxin Analysis in Food Products Using Immunoaffinity Extraction

  • Masahiko Takino
  • Hiroki Tanaka
  • Toshitsugu Tanaka
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 747)

Abstract

We developed a method for the simultaneous determination of deoxynivalenol, T-2 toxin, HT-2 toxin and zearalenone in wheat and biscuit by liquid chromatography/electrospray ionization/tandem mass spectrometry coupled with immunoaffinity extraction. This chapter describes a method to extract, clean-up, and quantitate these mycotoxins and the effect of the ion suppression of multifunctional column and IAC in the clean-up were compared.

Key words

Immunoaffinity column Liquid-chromatography Tandem mass-spectrometry 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Masahiko Takino
    • 1
  • Hiroki Tanaka
  • Toshitsugu Tanaka
  1. 1.Agilent TechnologiesHachiojiJapan

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