Abstract
This chapter describes a method for the formation of novel protein–nanotube hybrid conjugates. Specifically, we took advantage of the self-assembly and self-recognition properties of tubulin cytoskeletal protein immobilized onto carbon nanotubes to form nanotube-based biohybrids. Further biohybrid hierarchical integration in assemblies enabled molecular-level manipulation on engineered surfaces, as demonstrated with biocatalyst kinesin 1 ATPase molecular motor. The method presented herein can be extended for the preparation of biocatalyst-based or protein-based assemblies to be used as sensors or biological templates for nanofabrication.
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Acknowledgments
This work was supported by an NSF Nanoscale Science and Engineering Center (DMR 0642573). We thank Stefan Diez (Max-Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany) for providing kinesin and Douglas B. Chrisey (Department of Materials Science and Engineering, Rensselaer Polytechnic Institute) for the fluorescence microscopy facility.
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Dinu, C.Z., Bale, S.S., Dordick, J.S. (2011). Kinesin I ATPase Manipulates Biohybrids Formed from Tubulin and Carbon Nanotubes. In: Wang, P. (eds) Nanoscale Biocatalysis. Methods in Molecular Biology, vol 743. Humana Press. https://doi.org/10.1007/978-1-61779-132-1_7
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DOI: https://doi.org/10.1007/978-1-61779-132-1_7
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