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Nanoscale Biocatalysis pp 37–49Cite as

Molecular Assembly-Assisted Biocatalytic Reactions in Ionic Liquids

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 743))

Abstract

Room temperature ionic liquids (RTILs), having no measurable vapor pressure, represent an interesting class of tunable designer solvents. Due to their many unique properties, ILs have been used as attractive alternatives to environmentally harmful ordinary organic solvents in a wide range of applications including enzymatic biotransformation. Compared to conventional organic solvents, ILs offer many advantages for biocatalysis such as enhanced conversion rates, high enantioselectivity, better enzyme stability, and improved catalyst recoverability and recyclability. However, biocatalysis in ILs has not yet fully achieved its potential because many biocatalysts are insoluble in most ILs. This limitation could be overcome by the formation of nano/micrometer-sized aqueous microemulsion droplets in an IL continuous phase (referred to as water-in-IL microemulsions) stabilized by a layer of surfactants. Enzymes can be dissolved in such water droplets and protected from the unfavorable effect of ILs by the surfactant layer. In this chapter, a simple and effective method for the development of aqueous microemulsion droplets in a hydrophobic IL comprising an anionic surfactant sodium bis(2-ethyl-1-hexyl) sulfosuccinate (AOT) is presented. For this approach, we have synthesized a hydrophobic IL [C8mim][Tf2N] (1-octyl-3-methyl imidazolium bis(trifluoromethyl sulfonyl) amide) containing a long pendant hydrocarbon chain to facilitate the dissolution of AOT molecules. A detailed description of the procedure for the potential use of this newly developed water-in-IL reverse microemulsion for biocatalysis is also included.

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Acknowledgments

We gratefully acknowledge the JSPS (Japan Society for the Promotion of Science) for a JSPS Postdoctoral Fellowship (M. Moniruzzaman) and the necessary funding for this work. The authors would like to thank Prof. N. Kamiya for very helpful discussions.

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Authors and Affiliations

  1. Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka, 819-0395, Japan

    Muhammad Moniruzzaman & Masahiro Goto

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  1. Muhammad Moniruzzaman
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  2. Masahiro Goto
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Correspondence to Masahiro Goto .

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Editors and Affiliations

  1. Biotechnology Institute, Bioproducts & Biosystems Engineering, University of Minnesota, Folwell Ave. 2004, St. Paul, 55108, Minnesota, USA

    Ping Wang

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Moniruzzaman, M., Goto, M. (2011). Molecular Assembly-Assisted Biocatalytic Reactions in Ionic Liquids. In: Wang, P. (eds) Nanoscale Biocatalysis. Methods in Molecular Biology, vol 743. Humana Press. https://doi.org/10.1007/978-1-61779-132-1_4

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  • DOI: https://doi.org/10.1007/978-1-61779-132-1_4

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-131-4

  • Online ISBN: 978-1-61779-132-1

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