Methods to Study Mitotic Homologous Recombination and Genome Stability

  • Xiuzhong Zheng
  • Anastasiya Epstein
  • Hannah L. KleinEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 745)


Spontaneous mitotic recombination occurs in response to DNA damage incurred during DNA replication or from lesions that do not block replication but leave recombinogenic substrates such as single-stranded DNA gaps. Other types of damages result in general genome instability such as chromosome loss, chromosome fragmentation, and chromosome rearrangements. The genome is kept intact through recombination, repair, replication, checkpoints, and chromosome organization functions. Therefore when these pathways malfunction, genomic instabilities occur. Here we outline some general strategies to monitor a subset of the genomic instabilities: spontaneous mitotic recombination and chromosome loss, in both haploid and diploid cells. The assays, while not inclusive of all genome instability assays, give a broad assessment of general genome damage or inability to repair damage in various genetic backgrounds.

Key words

Genomic instability gene conversion chromosome loss mitotic recombination cell division 


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Xiuzhong Zheng
    • 1
  • Anastasiya Epstein
    • 1
  • Hannah L. Klein
    • 1
    Email author
  1. 1.Department of BiochemistryNew York University School of MedicineNew YorkUSA

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