Identification of GPCR Localization in Detergent Resistant Membranes
Lipid domains of the plasma membrane were originally described as a cell matrix insoluble in cold nonionic detergents and enriched in glycosphingolipids. Because of these biochemical properties, these membrane domains were termed detergent-resistant membranes (DRMs) or detergent-insoluble glycolipid-enriched (DIG) membranes. Membrane rafts and caveolae are two types of lipid domains that share these properties, as well as structural/functional dependence on membrane cholesterol. Membrane rafts and caveolae are believed to act as signaling platforms for ligand-activated receptors, thereby contributing to the regulation of receptor function. Here we describe a simple method to assess the association of GPCRs with detergent resistant membranes in native brain tissue and cultured cells.
Key wordsGPCR mGluR Membrane rafts Caveolae Lipid domains Detergent resistant membranes DRM DIG Density gradients Flotation
Supported by the U.S. National Institute of Health/NIMH (MH082870 to A.F.)
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