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Introduction to Section II: RNA Methods to Approach CFTR Expression

  • Ann HarrisEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 741)

Abstract

In this section, we review methods for the analysis of the CFTR gene and its transcript. First, we discuss techniques to accurately measure levels of CFTR mRNA in primary human cells; next, protocols for measuring CFTR transcripts that contain premature termination codons and for evaluating the role of nonsense-mediated decay which targets these transcripts; a further chapter considers methodology to investigate pre-mRNA splicing. The penultimate chapter concentrates on methods for evaluating microRNA regulation of gene expression in the context of airway disease. The final chapter considers methods to evaluate the chromatin structure of the active CFTR locus and to analyse the cis-acting regulatory elements that control it

Key words

CFTR gene mRNA levels pre-mRNA splicing nonsense-mediated decay microRNA chromatin structure regulatory elements 

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Human Molecular Genetics ProgramChildren’s Memorial Research Center, Northwestern University Feinberg School of MedicineChicagoUSA
  2. 2.Department of PediatricsNorthwestern University Feinberg School of MedicineChicagoUSA

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