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Analysis of CFTR Interactome in the Macromolecular Complexes

  • Chunying LiEmail author
  • Anjaparavanda P. Naren
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 741)

Abstract

Cystic fibrosis transmembrane conductance regulator (CFTR) is a chloride channel localized primarily at the apical surface of epithelial cells lining the airway, gut, exocrine glands, etc., where it is responsible for transepithelial salt and water transport. A growing number of proteins have been reported to interact directly or indirectly with CFTR chloride channel, suggesting that CFTR might regulate the activities of other ion channels, receptors, and transporters, in addition to its role as a chloride conductor. Most interactions occur primarily between the opposing terminal tails (N or C) of CFTR and its binding partners, either directly or mediated through various PDZ domain-containing proteins. This chapter describes methods we developed to cross-link CFTR into a macromolecular complex to identify and analyze the assembly and regulation of CFTR-containing complexes in the plasma membrane.

Key words

CFTR cross-linking macromolecular complex PDZ protein interacting partner 

Notes

Acknowledgments

We thank Dr David Armbruster for critically reading the manuscript and Ms. Feng Zhou for formatting the references. This work was supported by National Institute of Diabetes and Digestive and Kidney Diseases grants DK058545 and DK074996 (to A.P.N.), and American Heart Association (Midwest Affiliate) Beginning-grant-in-aid #0765185B (to C.L.).

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Department of Biochemistry and Molecular BiologyWayne State University School of MedicineDetroitUSA
  2. 2.Department of PhysiologyUniversity of Tennessee Health Science CenterMemphisUSA

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