Impact of MicroRNA in Normal and Pathological Respiratory Epithelia

  • Lisa Giovannini-Chami
  • Nathalie Grandvaux
  • Laure-Emmanuelle Zaragosi
  • Karine Robbe-Sermesant
  • Brice Marcet
  • Bruno Cardinaud
  • Christelle Coraux
  • Yves Berthiaume
  • Rainer Waldmann
  • Bernard Mari
  • Pascal BarbryEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 741)


Extensive sequencing efforts, combined with ad hoc bioinformatics developments, have now led to the identification of 1222 distinct miRNAs in human (derived from 1368 distinct genomic loci) and of many miRNAs in other multicellular organisms. The present chapter is aimed at describing a general experimental strategy to identify specific miRNA expression profiles and to highlight the functional networks operating between them and their mRNA targets, including several miRNAs deregulated in cystic fibrosis and during differentiation of airway epithelial cells.

Key words

Lung microRNA cystic fibrosis cancer 



This work is supported by CNRS, “Vaincre la Mucoviscidose,” CHU of Nice, ANR-09-GENO-039, European Community (MICROENVIMET, FP7-HEALTH-F2-2008-201279), the Canceropole PACA, and the Association de Recherche contre le Cancer.


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Lisa Giovannini-Chami
    • 1
    • 2
  • Nathalie Grandvaux
    • 3
  • Laure-Emmanuelle Zaragosi
    • 1
  • Karine Robbe-Sermesant
    • 1
  • Brice Marcet
    • 1
  • Bruno Cardinaud
    • 1
  • Christelle Coraux
    • 4
  • Yves Berthiaume
    • 5
  • Rainer Waldmann
    • 1
  • Bernard Mari
    • 1
  • Pascal Barbry
    • 1
    Email author
  1. 1.CNRS, Université de Nice Sophia Antipolis, IPMC, UMR6097Sophia AntipolisFrance
  2. 2.Service de Pédiatrie, Unité de Pneumo-AllergologieCHU de NiceFrance
  3. 3.Centre de Recherche du Centre Hospitalier de l’Université de Montréal, Université de Montréal, Hôpital Saint-Luc, PEAMontréalCanada
  4. 4.INSERM UMRS 903, CHU Maison BlancheReimsFrance
  5. 5.Centre de Recherche du Centre Hospitalier de l’Université de Montréal, Université de Montréal, Hôtel DieuMontréalCanada

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