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Neuroproteomics pp 13–26Cite as

Dissection of Rodent Brain Regions

Part of the Neuromethods book series (NM,volume 57)

Abstract

Dissection of brain tissue is an important step in sample preparation for (subcellular) proteomics studies. In this chapter, brain removal and separate dissection of multiple brain regions from a single brain are described in step-by-step protocol. This concerns dissection from fresh or frozen tissue of cerebellum, hippocampus, prefrontal cortex, and striatum.

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References

  1. Zhuo M, Hawkins RD (1995): Long-term depression: a learning-related type of synaptic plasticity in the mammalian central nervous system. Rev Neurosci 6:259–277.

    PubMed  CAS  Google Scholar 

  2. Teyler TJ, Discenna P (1984): Long-term potentiation as a candidate mnemonic device. Brain Res 319:15–28.

    PubMed  CAS  Google Scholar 

  3. Jorntell H, Hansel C (2006): Synaptic memories upside down: bidirectional plasticity at cerebellar parallel fiber-Purkinje cell synapses. Neuron 52:227–238.

    CrossRef  PubMed  Google Scholar 

  4. Olsen JV, Nielsen PA, Andersen JR, Mann M, Wisniewski JR (2007): Quantitative proteomic profiling of membrane proteins from the mouse brain cortex, hippocampus, and cerebellum using the HysTag reagent: mapping of neurotransmitter receptors and ion channels. Brain Res 1134:95–106.

    CrossRef  PubMed  CAS  Google Scholar 

  5. Trinidad JC, Thalhammer A, Specht CG, Lynn AJ, Baker PR, Schoepfer R, et al (2008): Quantitative analysis of synaptic phosphorylation and protein expression. Mol Cell Proteomics 7:684–696.

    PubMed  CAS  Google Scholar 

  6. Paxinos G, Franklin KBJ (2003): The mouse brain in stereotaxic coordinates: Academic press, San Diego.

    Google Scholar 

  7. Williams RW (1999): The Mouse Brain Library. http://www.mbl.org/atlas165/atlas165_start.html.

  8. Futterer CD, Maurer MH, Schmitt A, Feldmann RE, Jr., Kuschinsky W, Waschke KF (2004): Alterations in rat brain proteins after desflurane anesthesia. Anesthesiology 100:302–308.

    CrossRef  PubMed  Google Scholar 

  9. Snyder GL, Galdi S, Hendrick JP, Hemmings HC, Jr. (2007): General anesthetics selectively modulate glutamatergic and dopaminergic signaling via site-specific phosphorylation in vivo. Neuropharmacology 53:619–630.

    CrossRef  PubMed  CAS  Google Scholar 

  10. Westphalen RI, Hemmings HC, Jr. (2006): Volatile anesthetic effects on glutamate versus GABA release from isolated rat cortical nerve terminals: 4-aminopyridine-evoked release. J Pharmacol Exp Ther 316:216–223.

    CrossRef  PubMed  CAS  Google Scholar 

  11. Westphalen RI, Hemmings HC, Jr. (2006): Volatile anesthetic effects on glutamate versus GABA release from isolated rat cortical nerve terminals: basal release. J Pharmacol Exp Ther 316:208–215.

    CrossRef  PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, VU University, Amsterdam, The Netherlands

    Sabine Spijker

Authors
  1. Sabine Spijker
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Corresponding author

Correspondence to Sabine Spijker .

Editor information

Editors and Affiliations

  1. Ctr Neurogenomics/Cognitive Research, Dept Molecular & Cellular Neurobiology, VU University, De Boelelaan 1085, Amsterdam, 1081 HV, Netherlands

    Ka Wan Li

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© 2011 Springer Science+Business Media, LLC

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Spijker, S. (2011). Dissection of Rodent Brain Regions. In: Li, K. (eds) Neuroproteomics. Neuromethods, vol 57. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-111-6_2

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  • DOI: https://doi.org/10.1007/978-1-61779-111-6_2

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-110-9

  • Online ISBN: 978-1-61779-111-6

  • eBook Packages: Springer Protocols

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