Dissection of Rodent Brain Regions

  • Sabine SpijkerEmail author
Part of the Neuromethods book series (NM, volume 57)


Dissection of brain tissue is an important step in sample preparation for (subcellular) proteomics studies. In this chapter, brain removal and separate dissection of multiple brain regions from a single brain are described in step-by-step protocol. This concerns dissection from fresh or frozen tissue of cerebellum, hippocampus, prefrontal cortex, and striatum.

Key words

Dissection Mouse Brain Hippocampus Cerebellum Cortex 


  1. 1.
    Zhuo M, Hawkins RD (1995): Long-term depression: a learning-related type of synaptic plasticity in the mammalian central nervous system. Rev Neurosci 6:259–277.PubMedGoogle Scholar
  2. 2.
    Teyler TJ, Discenna P (1984): Long-term potentiation as a candidate mnemonic device. Brain Res 319:15–28.PubMedGoogle Scholar
  3. 3.
    Jorntell H, Hansel C (2006): Synaptic memories upside down: bidirectional plasticity at cerebellar parallel fiber-Purkinje cell synapses. Neuron 52:227–238.PubMedCrossRefGoogle Scholar
  4. 4.
    Olsen JV, Nielsen PA, Andersen JR, Mann M, Wisniewski JR (2007): Quantitative proteomic profiling of membrane proteins from the mouse brain cortex, hippocampus, and cerebellum using the HysTag reagent: mapping of neurotransmitter receptors and ion channels. Brain Res 1134:95–106.PubMedCrossRefGoogle Scholar
  5. 5.
    Trinidad JC, Thalhammer A, Specht CG, Lynn AJ, Baker PR, Schoepfer R, et al (2008): Quantitative analysis of synaptic phosphorylation and protein expression. Mol Cell Proteomics 7:684–696.PubMedGoogle Scholar
  6. 6.
    Paxinos G, Franklin KBJ (2003): The mouse brain in stereotaxic coordinates: Academic press, San Diego.Google Scholar
  7. 7.
    Williams RW (1999): The Mouse Brain Library.
  8. 8.
    Futterer CD, Maurer MH, Schmitt A, Feldmann RE, Jr., Kuschinsky W, Waschke KF (2004): Alterations in rat brain proteins after desflurane anesthesia. Anesthesiology 100:302–308.PubMedCrossRefGoogle Scholar
  9. 9.
    Snyder GL, Galdi S, Hendrick JP, Hemmings HC, Jr. (2007): General anesthetics selectively modulate glutamatergic and dopaminergic signaling via site-specific phosphorylation in vivo. Neuropharmacology 53:619–630.PubMedCrossRefGoogle Scholar
  10. 10.
    Westphalen RI, Hemmings HC, Jr. (2006): Volatile anesthetic effects on glutamate versus GABA release from isolated rat cortical nerve terminals: 4-aminopyridine-evoked release. J Pharmacol Exp Ther 316:216–223.PubMedCrossRefGoogle Scholar
  11. 11.
    Westphalen RI, Hemmings HC, Jr. (2006): Volatile anesthetic effects on glutamate versus GABA release from isolated rat cortical nerve terminals: basal release. J Pharmacol Exp Ther 316:208–215.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive ResearchVU UniversityAmsterdamThe Netherlands

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