Host Cells and Cell Banking

  • Glyn N. Stacey
  • Otto-Wilhelm Merten
Part of the Methods in Molecular Biology book series (MIMB, volume 737)


Gene therapy based on the use of viral vectors is entirely dependent on the use of animal cell lines, mainly of mammalian origin, but also of insect origin. As for any biotechnology product for clinical use, viral ­vectors have to be produced with cells derived from an extensively characterized cell bank to maintain the appropriate standard for assuring the lowest risk for the patients to be treated. Although many different cell types and lines have been used for the production of viral vectors, HEK293 cells or their derivatives have been extensively used for production of different vector types: adenovirus, oncorectrovirus, lentivirus, and AAV vectors, because of their easy handling and the possibility to grow them adherently in serum-containing medium as well as in suspension in serum-free culture medium. Despite this, these cells are not necessarily the best for the production of a given viral vector, and there are many other cell lines with significant advantages including superior growth and/or production characteristics, which have been tested and also used for the production of clinical vector batches. This chapter presents basic ­considerations concerning the characterization of cell banks, in the first part, and, in the second part, practically all cell lines (at least when public information was available) established and developed for the production of the most important viral vectors (adenoviral, oncoretroviral, lentiviral, AAV, baculovirus).

Key words

Cell bank Cell-line characterization Host cell lines Oncoretroviral vector Lentiviral vector Adeno-associated viral vector Adenoviral vector Baculovirus 






Adeno-associated virus


American Type Culture Collection


Baby hamster kidney (cell line)


Centers for Biologics Evaluation and Research



Cox 1

Cytochrome oxidase 1


Cumate operator


Dulbecco’s Modified Eagle Medium




DNAse-resistant particles


Deutsche Sammlung von Mikroorganismen und Zellkulturen: (German Resource Centre for Biological Material)

E1, E4

Early genes of adenovirus


Epstein-Barr virus


European Collection of Cell Cultures


European Medicine Agency


Fetal bovine serum


Fetal calf serum


Food and Drug Administration




Gibbon Ape leukemia Virus


Good cell culture practice


Green fluorescent protein


Gene of interest






Hepatitis B virus


Hepatitis C virus


Human embryonic kidney (cell line)


Human immunodeficiency virus


Human phosphoglycerate kinase promoter


Herpes simplex virus


International Conference on Harmonisation


Interlab Cell Line Collection


Infectious particle


Internal ribosomal entry sites


Inverted terminal repeat


Japanese Collection of Research Bioresources




Long terminal repeat


Master cell bank


Murine leukemia virus


Molony leukemia virus


Multiplicity of infection


Mustela putoris furo (ferret)


Murine Stem Cell Virus


National Institutes of Health


Open reading frame


Passage or promoter


Polymerase chain reaction


Replication-competent adenovirus


Replication-competent adeno-associated virus


Replication-competent lentivirus


Replication-competent retrovirus


Reverse transactivator (rtTA2S-M2) of the tetracycline (Tet)


Serum-free medium


Self-inactivating (vector)


Simian virus


Tetracyclin resistance


Thymidine kinase


Tn5 cell line


Transducing unit


Vesicular stomatitis virus


Vector genome


Working cell bank


Wild type


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© Springer Science+Business Media, LLC 2011 2011

Authors and Affiliations

  1. 1.GénéthonEvryFrance

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