Abstract
Maintenance of a mitotically inactive feeder layer which is able to provide extracellular matrix and growth factors can be critical in establishing and maintaining primary tumor cells. How feeder cells are handled and processed is crucial for providing trouble-free support for primary tumor cells and spontaneously immortalized lines.
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Acknowledgments
CP, SAW, and APS are funded by the Dystrophic Epidermolysis Bullosa Research Association. KJP is funded by Cancer Research UK.
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Pourreyron, C., Purdie, K.J., Watt, S.A., South, A.P. (2011). Feeder Layers: Co-culture with Nonneoplastic Cells. In: Cree, I. (eds) Cancer Cell Culture. Methods in Molecular Biology, vol 731. Humana Press. https://doi.org/10.1007/978-1-61779-080-5_37
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DOI: https://doi.org/10.1007/978-1-61779-080-5_37
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