Primary Cultures for Neurotoxicity Testing
Cell cultures are widely used in biomedical research. Primary cultures are directly obtained from fresh tissue and reproduce during days or weeks the major characteristics of the original tissue cells. Primary cell culture systems have shown their usefulness for studying the specific activities and the underlying mechanisms of a variety of test compounds. Brain primary cultures have become a powerful tool to analyze the toxic effects and mechanisms of potentially harmful agents in the different brain cell types.
Key wordsPrimary cultures Neurons Astrocytes Microglia Oligodendrocytes
The authors acknowledge support from the following grants: PI06/1212, PI08/1396, PI10/0453 and RD06/0013/1004 from ISCIII, SAF2006-13092-C02-02 and SAF2009-13093-C02-02 from MICINN, Spain, and LSHB-CT-2004-512051 from European Commission.
- 4.Hertz E, Yu ACH, Hertz L, Juurlink BHJ, Schousboe A (1989) Preparation of primary cultures of mouse cortical neurons. In: Shahar A, de Vellis J, Vernadakis A, Haber B (eds) A dissection and tissue culture manual of the nervous system. Alan R. Liss, New York, pp 203–206Google Scholar
- 5.Schousboe A, Meier E, Drejer J, Hertz L (1989) Preparation of primary cultures of mouse (rat) cerebellar granule cells. In: Shahar A, de Vellis J, Vernadakis A, Haber B (eds) A dissection and tissue culture manual of the nervous system. Alan R. Liss, New York, pp 203–206Google Scholar
- 11.Freshney RI (1992) Design and layout of the laboratory. In: Freshney RI (ed) Culture of animal cells, 3rd edn. Wiley-Lis, New York, pp 17–30Google Scholar