Primary Cultures for Neurotoxicity Testing

  • Carme Solà
  • Rosa Cristòfol
  • Cristina Suñol
  • Coral Sanfeliu
Part of the Neuromethods book series (NM, volume 56)


Cell cultures are widely used in biomedical research. Primary cultures are directly obtained from fresh tissue and reproduce during days or weeks the major characteristics of the original tissue cells. Primary cell culture systems have shown their usefulness for studying the specific activities and the underlying mechanisms of a variety of test compounds. Brain primary cultures have become a powerful tool to analyze the toxic effects and mechanisms of potentially harmful agents in the different brain cell types.

Key words

Primary cultures Neurons Astrocytes Microglia Oligodendrocytes 



The authors acknowledge support from the following grants: PI06/1212, PI08/1396, PI10/0453 and RD06/0013/1004 from ISCIII, SAF2006-13092-C02-02 and SAF2009-13093-C02-02 from MICINN, Spain, and LSHB-CT-2004-512051 from European Commission.


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Carme Solà
  • Rosa Cristòfol
  • Cristina Suñol
  • Coral Sanfeliu
    • 1
  1. 1.Institut d’Investigacions Biomèdiques de BarcelonaCSIC-IDIBAPSBarcelonaSpain

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