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Nucleic Acids Extraction from Laser Microdissected FFPE Tissue Sections

  • Renate BurgemeisterEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 724)

Abstract

Tissue heterogeneity is a common source of unsuccessful experiments. Laser capture microdissection is a tool to prepare homogeneous tissue and cell areas as starting material for reliable and reproducible results as it allows the defined investigation of spatially different tissue areas.

Nearly all samples allow the extraction of DNA. Fresh or fresh frozen samples are an ideal source for getting access to high-quality RNA. But also the large archives of formalin-fixed, paraffin-embedded (FFPE) tissue specimens are a valuable source of sample material for RNA extraction. Optimized protocols may help to make the RNA from FFPE material suitable for expression studies.

Key words

Laser microdissection Defined tissue areas Homogeneous tissue Biomarker identification High-quality RNA FFPE tissue Expression studies Archived biopsies 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Carl Zeiss MicroImagingMünchenGermany

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