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Automated Analysis of FISH-Stained HER2/neu Samples with Metafer

  • Christian SchunckEmail author
  • Eiman Mohammad
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 724)

Abstract

The HER2/neu gene (also known as ERBB2) is located on chromosome 17 (q11.2–q12) and encodes a glycoprotein known to be a member of the epidermal growth factor receptor family. Clinically, the determination of its amplification status is of utmost importance, as 10–35% of invasive human breast carcinomas come along with HER2/neu overexpression, and treatment has to be adjusted accordingly. Here a method to analyze HER2 FISH samples with digital microscopy, using the slide scanning ­platform Metafer PV (MetaSystems, Altlussheim, Germany), is presented. Metafer PV is a system for the automation of HER2/neu FISH assay analysis of samples hybridized with the Abbott PathVysion® probe kit.

Key words

HER2/neu Breast cancer Metafer PV Digital microscopy PathVysion® Tile sampling Slide scanning 

References

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.MetaSystemsAltlussheimGermany
  2. 2.Faculty of Medicine, Molecular Pathology UnitKuwait UniversitySafatKuwait

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