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An Alternative Fixative to Formalin Fixation for Molecular Applications: The RCL2®-CS100 Approach

  • Amélie Denouël
  • Florence Boissière-Michot
  • Philippe Rochaix
  • Frédéric Bibeau
  • Nathalie BoulleEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 724)

Abstract

Molecular analysis of tissue lesions is increasingly used in laboratories to identify new prognostic and therapeutic markers. Formalin has long been the tissue fixative of choice in the laboratories of pathology, as it preserves tissue morphology allowing accurate histological diagnosis. However, formalin is highly toxic and alters nucleic acids and protein integrity, so that new fixatives are critically needed that would allow both morphological and molecular analysis on the same tissue specimen. Recently, we found RCL2®-CS100, a noncross-linking fixative, to display interesting performances regarding tissue morphology and DNA, RNA, and protein quality. We adapted RCL2 tissue fixation protocol so it could be used on a routine and automated laboratory basis, still preserving its good performances. This protocol will be described in detail in the following review.

Key words

Tissue fixation Formalin RCL2®-CS100 Immunohistochemistry Nucleic acids DNA RNA 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Amélie Denouël
    • 1
  • Florence Boissière-Michot
    • 1
  • Philippe Rochaix
    • 2
  • Frédéric Bibeau
    • 1
  • Nathalie Boulle
    • 3
    Email author
  1. 1.CRLC Val d’AurelleMontpellierFrance
  2. 2.CRLC Claudius RegaudToulouseFrance
  3. 3.Hospital Arnaud de VilleneuveMontpellierFrance

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