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Surface Modification and Biomolecule Immobilization on Polymer Spheres for Biosensing Applications

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 726)

Abstract

Microspheres and nanospheres are being used in many of today’s biosensing applications for automated sample processing, flow cytometry, signal amplification in microarrays, and labeling in multiplexed analyses. The surfaces of the spheres/particles need to be modified with proteins and other biomolecules to be used in these sensing applications. This chapter contains protocols to modify carboxyl- and amine-coated polymer spheres with proteins and peptides.

Key words

Microspheres Nanoparticles Immobilization Latex spheres 
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Notes

Acknowledgments

This work was supported by the Defense Threat Reduction Agency and ONR/NRL 6.2 work unit 6336. The views are those of the authors and do not represent opinion or policy of the Naval Research Laboratory, US Navy, or Department of Defense.

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© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Center for Bio/Molecular Science and EngineeringU.S. Naval Research LaboratoryWashingtonUSA

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