Abstract
Semiconductor photocatalysis using nanoparticulate TiO2 has proven to be a promising technology for use in catalytic reactions, in the cleanup of water contaminated with hazardous industrial by-products, and in nanocrystalline solar cells as a photoactive material. Metal oxide semiconductor colloids are of considerable interest because of their photocatalytic properties. The coordination sphere of the surface metal atoms is incomplete and thus traps light-induced charges, but also exhibits high affinity for oxygen-containing ligands and gives the opportunity for chemical modification. We use enediol linkers, such as dopamine and its analogs, to bridge the semiconductors to biomolecules such as DNA or proteins. Nanobio hybrids that combine the physical robustness and chemical reactivity of nanoscale metal oxides with the molecular recognition and selectivity of biomolecules were developed. Control of chemical processes within living cells was achieved using TiO2 nanocomposites in order to develop new tools for advanced nanotherapeutics. Here, we describe general experimental approaches for synthesis and characterization of high crystallinity, water soluble 5 nm TiO2 particles and their nanobio composites, methods of cellular sample preparation for advanced Synchrotron-based imaging of nanoparticles in single cell X-ray fluorescence, and a detailed experimental setup for application of the high-performance TiO2-based nanobio photocatalyst for targeted lysis of cancerous or other disordered cells.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Thurnauer, M. C., Dimitrijevic, N. M., Poluektov, O. G., and Rajh, T. (2004) Photoinitiated charge separation: from photosynthesis to nanoparticles. Spectrum 17, 10–15.
Liu, J., de la Garza, L., Zhang, L., Dimitrijevic, N. M., Zuo, X. B., Tiede, D. M., et al. (2007) Photocatalytic probing of DNA sequence by using TiO2/dopamine-DNA triads. Chem. Phys. 339, 154–163.
Liu, J., Saponjic, Z. V., Dimitrijevic, N. M., Luo, S., Preuss, D., and Rajh, T. (2006) Hybrid TiO2 nanoparticles: an approach for developing artificial restriction enzymes. in: Colloidal Quantum Dots for Biomedical Applications (Osinski, M., Yamamoto, K., and Jovin, T. M. eds.). Bellingham, WA. SPIE Proc Series 6096, 60960F.
Rozhkova, E. A., Ulasov, I. V., Lai, B., Dimitrijevic, N. M., Lesniak, M. S., and Rajh, T. (2009) A high-performance nanobio photocatalyst for targeted brain cancer therapy. Nano Lett. 9, 3337–3342.
Dimitrijevic, N. M., Rozhkova, E. A., and Rajh, T. (2009) Dynamics of localized charges in dopamine-modified TiO2 and their effect on the formation of reactive oxygen species. J. Am. Chem. Soc. 131, 2893–2899.
Rajh, T., Chen, L. X., Lukas, K., Liu, T., Thurnauer, M. C., and Tiede, D. M. (2002) Surface restructuring of nanoparticles: an efficient route for ligand-metal oxide crosstalk. J. Phys. Chem. B 106, 10543–10552.
Redfern, P. C., Zapol, P., Curtiss, L. A., Rajh, T., and Thurnauer, M. C. (2003) Computational studies of catechol and water interactions with titanium oxide nanoparticles. J. Phys. Chem. B 107, 11419–11427.
Saponjic, Z. V., Dimitrijevic, N. M., Tiede, D. M., Goshe, A. J., Zuo, X., Chen, L. X., et al. (2005) Shaping nanometer-scale architecture through surface chemistry. Adv. Mater. 17, 965–971.
Rajh, T., Saponjic, Z., Liu, J., Dimitrijevic, N. M., Scherer, N. F., Vega-Arroyo, M., et al. (2004) Charge transfer across the nanocrystalline-DNA interface: probing DNA recognition. Nano Lett. 4, 1017–1023.
Dimitrijevic, N. M., Saponjic, Z. V., Rabatic, B. M., and Rajh, T. (2005) Assembly and charge transfer in hybrid TiO2 architectures using biotin-avidin as a connector. J. Am. Chem. Soc. 127, 1344–1345.
Paunesku, T., Rajh, T., Wiederrecht, G., Maser, J., Vogt, S., Stojicevic, N., et al. (2003) Biology of TiO2-oligonucleotide nanocomposites. Nat. Mater. 2, 343–346.
de la Garza, L., Saponjic, Z. V., Dimitrijevic, N. M., Thurnauer, M. C., and Rajh, T. (2006) Surface states of titanium dioxide nanoparticles modified with enediol ligands. J. Phys. Chem. B 110, 680–686.
Rajh, T., Dimitrijevic, N. M., Elhofy, A., and Rozhkova, E. A. (2010) Hybrid TiO2 based nanocomposites-new tools for biotechnology and biomedicine. in: Handbook of Nanophysics: Functional Nanomaterials (Sattler, K. D. ed.). CRC, Boca Raton, FL.
Rajh, T., Ostafin, A. E., Micic, O. I., Tiede, D. M., and Thurnauer, M. C. (1996) Surface modification of small particle TiO2 colloids with cysteine for enhanced photochemical reduction: an EPR study. J. Phys. Chem. 100, 4538–4545.
Nakamura, F. (2001) Biochemical, electron microscopic and immunohistological observations of cationic detergent-extracted cells: detection and improved preservation of microextensions and ultramicroextensions. BMC Cell Biol. 2, 10.
Finney, L., Mandava, S., Ursos, L., Zhang, W., Rodi, D., Vogt, S., et al. (2007) X-ray fluorescence microscopy reveals large-scale relocalization and extracellular translocation of cellular copper during angiogenesis. Proc. Natl. Acad. Sci. USA. 104, 2247–2252.
Committee on Prudent Practices for Handling, Storage, and Disposal of Chemicals in Laboratories, National Research Council. (1995) Prudent Practices in the Laboratory Handling and Disposal of Chemicals. National Academy Press, Washington, DC.
Acknowledgments
Work at the Center for Nanoscale Materials was supported by the US Department of Energy, Office of Science, Office of Basic Energy Sciences, under Contract No. DE-AC02-06CH11357. We are thankful to our colleagues Drs. B. Lai, L. Finney, S. Vogt and J. Maser from Advanced Photon Source, Argonne National Laboratory and collaborators Drs. M. S. Lesniak and I. V. Ulasov from the University of Chicago, Brain Tumor Center.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2011 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Rajh, T., Dimitrijevic, N.M., Rozhkova, E.A. (2011). Titanium Dioxide Nanoparticles in Advanced Imaging and Nanotherapeutics. In: Hurst, S. (eds) Biomedical Nanotechnology. Methods in Molecular Biology, vol 726. Humana Press. https://doi.org/10.1007/978-1-61779-052-2_5
Download citation
DOI: https://doi.org/10.1007/978-1-61779-052-2_5
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-61779-051-5
Online ISBN: 978-1-61779-052-2
eBook Packages: Springer Protocols