Abstract
Applications in a variety of fields rely on the high-throughput ultrasensitive and multiplexed detection of oligonucleotides. However, the conventional microarray-based techniques that employ fluorescent dyes are hampered by several limitations; they require target amplification, fluorophore labeling, and complicated instrumentation, while the fluorophore-labeled species themselves exhibit slow binding kinetics, photo-bleaching effects, and overlapping spectral profiles. Among the emerging nanomaterials that are being used to solve these problems, oligonucleotide–gold nanoparticle conjugates (Oligo-AuNPs) have recently been highlighted due to their unique chemical and physical properties. In this chapter, a detection scheme for oligonucleotides that utilize Oligo-AuNPs is evaluated with multiple oligonucleotide targets. This scheme takes advantage of the sharp melting transitions, intense optical properties, catalytic properties, enhanced binding properties, and the programmable assembly/disassembly of Oligo-AuNPs.
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Lee, JS. (2011). Multiplexed Detection of Oligonucleotides with Biobarcoded Gold Nanoparticle Probes. In: Hurst, S. (eds) Biomedical Nanotechnology. Methods in Molecular Biology, vol 726. Humana Press. https://doi.org/10.1007/978-1-61779-052-2_2
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DOI: https://doi.org/10.1007/978-1-61779-052-2_2
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