Abstract
A tremendous amount of information regarding the nature and regulation of heterochromatin has emerged in the past 10 years. This rapid progress is largely due to the development of techniques such as chromatin immunoprecipitation or “ChIP,” which allow analysis of chromatin structure. Further technological advances such as microarray analysis and, more recently, deep sequencing technologies, have made ChIP an even more powerful tool. ChIP allows the investigator to identify protein interactions and/or the presence of various chromatin modifications at specific genomic loci.
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Acknowledgments
The authors would like to thank Michele McDonough for helpful comments on the manuscript. T.V. and J.D. are supported by the NIH (R01 GM074986) and the generous support of the Robert H. Lurie Comprehensive Cancer Center.
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Volpe, T.A., DeMaio, J. (2011). Chromatin Immunoprecipitation in Fission Yeast. In: Hobman, T., Duchaine, T. (eds) Argonaute Proteins. Methods in Molecular Biology, vol 725. Humana Press. https://doi.org/10.1007/978-1-61779-046-1_2
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DOI: https://doi.org/10.1007/978-1-61779-046-1_2
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