Antiviral RNAi pp 339-353 | Cite as

Antibody-Mediated Delivery of siRNAs for Anti-HIV Therapy

  • Sang-Soo Kim
  • Sandesh Subramanya
  • Dan Peer
  • Motomu Shimaoka
  • Premlata Shankar
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 721)

Abstract

RNA interference (RNAi) is a potent and specific gene silencing mechanism that utilizes small ­double-stranded RNA intermediates (small interfering RNAs or siRNAs) to target homologous mRNA sequences for degradation. The therapeutic potential of RNAi for HIV infection has been demonstrated in many studies. However, successful clinical application of RNAi is contingent on developing practical strategies to deliver siRNA to the desired target cells and tissues. Recently, there has been significant progress towards developing reagents that selectively deliver exogenous siRNA to immune cells that are targeted by HIV or involved in viral pathogenesis, such as T cells, macrophages, and dendritic cells. Here, we describe details of two antibody-based strategies for systemic delivery of siRNA either specifically to T cells via the CD7 receptor or to multiple immune cell types via LFA-1, present on all leukocytes.

Key words

RNA interference Small interfering RNA Targeted delivery Cationic peptide Liposome scFvCD7 Integrin LFA-1 (lymphocyte function-associated antigen-1) I-tsNPs (integrin-targeted stabilized nanoparticles) HIV 

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Copyright information

© Humana Press 2011

Authors and Affiliations

  • Sang-Soo Kim
  • Sandesh Subramanya
  • Dan Peer
  • Motomu Shimaoka
  • Premlata Shankar
    • 1
  1. 1.Department of Biomedical Sciences, Center of Excellence for Infectious Diseases, Paul L. Foster School of MedicineTexas Tech University Health Sciences CenterEl PasoUSA

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