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Screening and Characterization of Plant Cell Walls Using Carbohydrate Microarrays

  • Iben Sørensen
  • William G. T. Willats
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 715)

Abstract

Plant cells are surrounded by cell walls built largely from complex carbohydrates. The primary walls of growing plant cells consist of interdependent networks of three polysaccharide classes: cellulose, cross-linking glycans (also known as hemicelluloses), and pectins. Cellulose microfibrils are tethered together by cross-linking glycans, and this assembly forms the major load-bearing component of primary walls, which is infiltrated with pectic polymers. In the secondary walls of woody tissues, pectins are much reduced and walls are reinforced with the phenolic polymer lignin. Plant cell walls are essential for plant life and also have numerous industrial applications, ranging from wood to nutraceuticals. Enhancing our knowledge of cell wall biology and the effective use of cell wall materials is dependent to a large extent on being able to analyse their fine structures. We have developed a suite of techniques based on microarrays probed with monoclonal antibodies with specificity for cell wall components, and here we present practical protocols for this type of analysis.

Key words

Carbohydrate microarrays Plant cell walls Polysaccharides Antibodies CoMPP 

References

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    Moller, I., Sørensen, I., Bernal, A. J., Blaukopf, C., Lee, K., Øbro, J., Roberts, A., Mikkelsen, J. D., Knox, J. P., Bacic, A. and Willats, W. G. T. (2007) High-throughput mapping of cell-wall polymers between and within plants using novel microarrays. Plant J 50, 1118–1128.PubMedCrossRefGoogle Scholar
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    Sørensen, I., Pettolino, F. A., Wilson, S. M., Doblin, S. M., Johansen, B., Bacic, A. and Willats, W. G. T. (2008) Mixed linkage (1→3),(1→4)-β-d-glucan is not unique to the Poales and is an abundant component of Equisetum arvense cell walls. Plant J 54, 510–521.PubMedCrossRefGoogle Scholar
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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Iben Sørensen
    • 1
  • William G. T. Willats
    • 1
  1. 1.Department of BiologyUniversity of CopenhagenCopenhagenDenmark

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