Carbohydrate Gel Electrophoresis

  • Florence GoubetEmail author
  • Paul Dupree
  • Katja Salomon Johansen
Part of the Methods in Molecular Biology book series (MIMB, volume 715)


Polysaccharide analysis using carbohydrate gel electrophoresis (PACE) relies on derivatization of the reducing ends of sugars with a fluorophore, followed by electrophoresis under optimized conditions in polyacrylamide gels. PACE is a sensitive and simple tool for studying polysaccharide structure or quantity and also has applications in the investigation of enzyme specificity.

Key words

Oligosaccharide Monosaccharide Polysaccharide Pectin Hemicellulose Enzyme 


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Florence Goubet
    • 1
    Email author
  • Paul Dupree
    • 2
  • Katja Salomon Johansen
    • 3
  1. 1.Bayer BioScience NVGhentBelgium
  2. 2.Department of BiochemistryUniversity of CambridgeCambridgeUK
  3. 3.Novozymes A/SBagsvaerdDenmark

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